苏州城区河道大型水生生物分布特征

为研究苏州城区河道大型水生生物时空分布特征,于2020年5月开始,对苏州古城区主要河道23个断面的大型水生生物及水质指标进行为期一年监测,分析其理化因子时空分布差异,并探讨与大型水生生物相关的影响因素,体现苏州河道恢复程度。结果表明,水温、pH、溶解性总固体、电导率、溶解氧、透明度与氨氮在不同月份间呈显著性差异（P<0.05）,总氮和总磷在不同月份间具有极显著性差异（P<0.01）。研究期间苏州城市河道中共检出5种沉水植物,10种大型底栖无脊椎动物,7种鱼类。其中沉水植物辛普森指数最高,结构最均匀,各断面中沧浪亭桥玛格列夫指数最高,物种最丰富。有无沉水植物分组间流速呈显著性差异,总氮、氨氮存在极显著差异。大型底栖无脊椎动物有无的河道在流速、水深、透明度和溶解氧存在极显著性差异。按是否存在鱼类对比,水质指标均无显著性差异。冗余分析（RDA）结果也表明,氨氮、水温、溶解氧和透明度是影响城区河道大型水生生物分布的主要水质指标。     

城市人工湖泊浮游植物群落结构特征及其主要环境因子分析——以长沙梅溪湖为例

长沙梅溪湖为新兴城市人工湖泊,是国家级新区湘江新区的城市“海绵体”,其水环境与水生态状况是该区域高质量发展关注的重点,对维护湘江新区生态环境具有重要意义。为揭示梅溪湖水生态现状,分季度采集梅溪湖水样,分析浮游植物群落特征及其与环境因子的关系。结果显示梅溪湖浮游植物共有7门96种,其中以绿藻、硅藻和蓝藻种类数居多,占总种类数的86.46%,主要优势种为细小隐球藻（Aphanocapsa elacjista）、小环藻（Cyclotella sp.）、链状曲壳藻（Achnanthes catenata）和尖尾蓝隐藻（Chroomonas acuta）,优势种具有明显季节交替性。浮游植物年均密度为（23.97±9.95）×10^6 cells/L,各季度东部水域密度均高于西部水域;多样性指数评价结果表明梅溪湖水体处于中度污染和中-富营养状态,与水质评价结果基本一致。浮游植物与环境因子基于转化的冗余分析结果显示,水温、氧化还原电位、总氮和亚硝酸盐氮是影响梅溪湖浮游植物群落的主要驱动因子,控制总氮等营养盐的输入将是改善梅溪湖水质和营养状态的有效手段。     

中国野桑蚕人工饲料育研究初报

为解决野桑蚕作为试验昆虫在室内饲养难的问题,以江苏启东野桑蚕为材料,用家蚕低成本饲料进行了饲育研究。在催青期温度25℃、饲育温度27~25℃以及光照条件1~2龄12 L 12 D、3~5龄15 L 9 D的条件下,野桑蚕人工饲料育的2龄起蚕率为31.3%,幼虫期20~48 d,雌、雄蛹体质量分别为0.45、0.25 g,蛹期17~23 d,雌蛾平均产卵266粒,产滞育卵的母蛾占45.0%。研究结果表明,人工饲料育能够使野桑蚕顺利完成世代发育。     

MDV Meq-clustered miRNAs基因缺失BAC克隆株的构建及其体外增殖特性

为构建缺失马立克氏病病毒(Mareks disease virus,MDV)Meq基因簇microRNAs(Meq-clustered miRNAs的突变株,本研究在vv MDV GX0101细菌人工染色体(bacterial artificial chromosome,BAC)克隆的基础上,采用Red/ET同源重组技术将Meq-clustered miRNAs的编码基因进行缺失突变,经PCR鉴定及序列分析证明Meq-cluster miRNAs序列成功缺失后,提取Δmeq-miRNAs BAC DNA,转染鸡胚成纤维细胞(CEF)进行病毒拯救,用SYBR GreenⅠqRT-PCR检测病毒体外增殖特性。结果表明成功拯救出缺失MDV Meq-clustered miRNAs基因的感染性BAC克隆株GX0101Δmeq-miRNAs,且该缺失株与亲本株GX0101BAC具有相似的增殖曲线,Meq-clustered miRNAs是MDV体外复制的非必需基因。MDV Meq-clustered miRNAs基因缺失感染性BAC克隆株的成功构建为进一步研究MDV Meq-clustered miRNAs在MDV致病和致肿瘤方面的作用奠定了基础。     

冬季皓月×菁松全龄人工饲料育试验简报

2014年冬季,在如东县组织进行了皓月×菁松蚕品种全龄人工饲料育农村养蚕试验,生产的鲜茧上车率为75.91%,粒茧丝长为926.9m,解舒率仅45.28%,茧丝纤度为1.891D,光折为319kg。通过加强饲育管理和簇中管理,好蛹率上升,茧层含水率、公斤茧用料量有所下降。     

Effect of dietary Schizochytrium microalga oil and fish oil on plasma cholesterol level in rats

The purpose of the study was to test the hypothesis that the dietary oils with different content of n‐3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) affect plasma lipid level in rats in a different degree. The diets with 6% of fish oil (FO) and Schizochytrium microalga oil (SchO; EPA+DHA content in the diets 9.5 + 12.3 and 2.6 + 29.5% of the sum of total fatty acids, respectively) were used; the diet with 6% of safflower oil (high content of n‐6 PUFA linoleic acid, 65.5%; EPA+DHA content 0.7 + 0.9%) was used as a control. The difference between FO and SchO was established only in the case of plasma triacylglycerol (TAG) level: plasma TAG of the FO‐fed rats did not differ from the control rats (p > 0.05), while SchO decreased (p < 0.05) plasma TAG to 46% of the control. On the other hand, FO and SchO decreased (p < 0.05) total plasma cholesterol (TC) in rats in the same extent, to 73% of the control. Regarding the underlying mechanisms for the TC decrease, both SchO and FO up‐regulated hepatic Insig‐1 gene (181 and 133% of the control; p < 0.05), which tended (p = 0.15 and p = 0.19 respectively) to decrease the amount of hepatic nSREBP‐2 protein (61 and 66% of the control). However, neither SchO nor FO influenced hepatic 3‐hydroxy‐3‐methyl‐glutaryl‐CoA reductase gene expression (p > 0.05); SchO (but not FO) increased (p < 0.05) low‐density lipoprotein receptor mRNA in the liver. It was concluded that the decrease of total plasma cholesterol might be caused by an increased cholesterol uptake from plasma into the cells (in the case of SchO), but also by other (in the present study not tested) mechanisms.     

Fertility of Holstein cows and heifers submitted to timed artificial insemination and receiving one or two doses (12 h apart) of semen

The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.     

人工辅助措施对文冠果坐果率的影响

通过采取不同的人工辅助措施进行了提高文冠果坐果率的试验,结果表明：人工辅助措施中,以液体喷雾提高坐果率效果最为显著,坐果率达到4.12%。喷施植物生长调节剂中,以50 mg L-1的赤霉素处理,提高坐果率效果最显著,达到5.47%;其次为2000 mg L-1的丁酰肼处理水平,为4.95%;再次为1000 mg L-1的青鲜素处理水平,达到4.89%。     

白马鸡人工驯养初步研究

通过人工饲养的方式探索出白马鸡驯养的经验,即:在野生白马鸡适宜生境下建立饲养场,从育雏阶段开始进行人工投食饲养和本地土鸡带领相结合的驯养方式。目前已经完成从子一代到子二代的完整驯养、饲养流程,取得了云南省林业厅的驯养繁殖许可证。对育雏、亚成体、成体阶段的喂食量、存活情况等作了分析,认为接近原始生境的环境及本地带雏母鸡是成功的重要条件。     

沙地樟子松人工林冠下人工更新研究

在渐伐后郁闭度不足0.4的沙地樟子松人工林冠下进行人工更新试验,10年后观测各树种的生长状况,并对林下的土壤进行调查.结果表明:利用这种冠下人工更新方式使樟子松纯林转变为多树种混交的复层林;色木槭等4个树种可以作为冠下更新的主培树种;冠下人工更新可以逐渐改良土壤,延缓樟子松人工林林地土壤的衰退.